MeRIP-Seq (m6A-Seq)

Principle

MeRIP-Seq enriches modified RNA fragments with m6A-specific antibodies and uses sequencing and bioinformatics to map m6A-enriched regions. It compares modification patterns between conditions and examines their relationship with expression and phenotypes.

Applications

• Study factors involved in writing, removing or recognizing m6A
• Compare modifications during disease, development or treatment
• Combine expression data to prioritize modification-associated transcripts

Method features

• Profiles RNA modification enrichment transcriptome-wide
• Supports integration with input RNA expression data
• Enriched regions are the main readout; resolution depends on design

Workflow

1. 01

   Prepare RNA and retain input controls

2. 02

   Fragment RNA and perform m6A immunoenrichment

3. 03

   Prepare and sequence input and enriched libraries

4. 04

   Identify modified regions and integrate expression results

This is a workflow overview. Experimental and analytical details depend on sample conditions and research objectives.